Potential of Weed Extracts to Control Bacterial Blight Disease Caused by Xanthomonas oryzae pv. Oryzae in Rice. This study aimed to find out the potential and effectivity of weed extracts to control bacterial blight disease caused by Xanthomonas oryzae pv. oryzae in rice. The experimentation process has four stages. In preparing the medium, 300 g of sliced unpeeled potato was boiled and 24 g agar, 0.5 g K[4]HPO[4], 0.5 g CaCO[3], 0.5 g NaCl, 5 g peptone and 20 g sucrose were melted.
Potato water was added to the solution to make up 1 liter. The medium was dispensed in petri plates and flasks and sterilized. In the identification and isolation of bacteria, rice infected leaves were disinfected with 1% NaOCl and rinsed with running water. The leaves were cut aseptically, placed in sterilized glass slide, stained and placed in the medium. The leaves of the weeds were disinfected with 1% NaOCl, rinsed three time , pounded, filtered and sterilized. The extracts were pipetted to the cultured bacteria after 24 hour exposure to ultraviolet rays. One way analysis of variance was used in ten replicates and six treatments. The results rejected the null hypotheses and showed that the five extracts have the potential to control bacterial leaf blight disease caused by Xanthomonas oryzae pv. oryzae in rice. Treatment B, Cobra-cobra (Ipomea sp.) was the most effective among the five weeds. Therefore, weeds such as Lemon Grass (Cymbopogon citratus, Asyang (Mikania chordata), Bugang (Roetboella exaltata), Cobracobra (Ipomea sp. And Cogon (Imperata cylindrica) have the potential to control bacterial blight disease caused by Xanthomonas oryzae pv. oryzae in rice.
Materials & Equipment
Further clarification of the procedures and results should be directed to the researchers and adviser.
Researchers:
Shagini A. Amar
Adviser:
Mrs. Bernadette M. Suarez and Mrs. Adaiah A. Opeña
Doña Hortencia Salas Benedicto National High School